CHOmpact: A reduced metabolic model of Chinese hamster ovary cells with enhanced interpretability.

ABSTRACT: Metabolic modeling has emerged as a key tool for the characterization of biopharmaceutical cell culture processes. Metabolic models have also been instrumental in identifying genetic engineering targets and developing feeding strategies that optimize the growth and productivity of Chinese hamster ovary (CHO) cells. Despite their success, metabolic models of CHO cells still present considerable challenges. Genome-scale metabolic models (GeMs) of CHO cells are very large (>6000 reactions) and are difficult to constrain to yield physiologically consistent flux distributions. The large scale of GeMs also makes the interpretation of their outputs difficult. To address these challenges, we have developed CHOmpact, a reduced metabolic network that encompasses 101 metabolites linked through 144 re

SUBMITTER: Jimenez Del Val I 

PROVIDER: S-EPMC10952303 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature