Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay.

ABSTRACT: White-rot fungi employ secreted carbohydrate-active enzymes (CAZymes) along with reactive oxygen species (ROS), like hydrogen peroxide (H₂O₂), to degrade lignocellulose in wood. H₂O₂ serves as a co-substrate for key oxidoreductases during the initial decay phase. While the degradation of lignocellulose by CAZymes is well documented, the impact of ROS on the oxidation of the secreted proteins remains unclear, and the identity of the oxidized proteins is unknown. Methionine (Met) can be oxidized to Met sulfoxide (MetO) or Met sulfone (MetO₂) with potential deleterious, antioxidant, or regulatory effects. Other residues, like proline (Pro), can undergo carbonylation. Using the white-rot Pycnoporus cinnabarinus grown on aspen wood,

SUBMITTER: Molinelli L 

PROVIDER: S-EPMC10952391 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature