Culture-independent detection of low-abundant Clostridioides difficile in environmental DNA via PCR.

ABSTRACT: Clostridioides difficile represents a major burden to public health. As a well-known nosocomial pathogen whose occurrence is highly associated with antibiotic treatment, most examined C. difficile strains originated from clinical specimen and were isolated under selective conditions employing antibiotics. This suggests a significant bias among analyzed C. difficile strains, which impedes a holistic view on this pathogen. In order to support extensive isolation of C. difficile strains from environmental samples, we designed a detection PCR that targets the hpdBCA-operon and thereby identifies low abundances of C. difficile in environmental samples. This operon encodes the 4-hydroxyphenylacetate decarboxylase, which catalyzes the production of the an

SUBMITTER: Schuler MA 

PROVIDER: S-EPMC10952401 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature