Harnessing the endogenous Type I-C CRISPR-Cas system for genome editing in Bifidobacterium breve.

ABSTRACT: Bifidobacterium breve, one of the main bifidobacterial species colonizing the human gastrointestinal tract in early life, has received extensive attention for its purported beneficial effects on human health. However, exploration of the mode of action of such beneficial effects exerted by B. breve is cumbersome due to the lack of effective genetic tools, which limits its synthetic biology application. The widespread presence of CRISPR-Cas systems in the B. breve genome makes endogenous CRISPR-based gene editing toolkits a promising tool. This study revealed that Type I-C CRISPR-Cas systems in B. breve can be divided into two groups based on the amino acid sequences encoded by cas gene clusters. Deletion of the gene coding uracil phosphoribosyl-transferase

SUBMITTER: Han X 

PROVIDER: S-EPMC10952402 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature