Stimulation of platelet P2Y₁ receptors by different endogenous nucleotides leads to functional selectivity via biased signalling.

ABSTRACT:

Background and purpose

Platelet function during inflammation is dependent on activation by endogenous nucleotides. Non-canonical signalling via the P2Y₁ receptor is important for these non-thrombotic functions of platelets. However, apart from ADP, the role of other endogenous nucleotides acting as agonists at P2Y₁ receptors is unknown. This study compared the effects of ADP, Ap3A, NAD⁺ , ADP-ribose, and Up4A on platelet functions contributing to inflammation or haemostasis.

Experimental approach

Platelets obtained from healthy human volunteers were incubated with ADP, Ap3A, NAD⁺ , ADP-ribose, or Up4A, with aggregation and fibrinogen binding measured (examples of function during haemostasis) or before exposure to fMLP to measure pl

SUBMITTER: Arkless KL 

PROVIDER: S-EPMC10952403 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature