A multifaceted investigation of lactococcal strain diversity in undefined mesophilic starter cultures.

ABSTRACT: The dairy fermentation industry relies on the activity of lactic acid bacteria in robust starter cultures to accomplish milk acidification. Maintenance of the composition of these starter cultures, whether defined or undefined, is essential to ensure consistent and high-quality fermentation end products. To date, limited information exists regarding the microbial composition of undefined starter culture systems. Here, we describe a culture-based analysis combined with a metagenomics approach to evaluate the composition of two undefined mesophilic starter cultures. In addition, we describe a qPCR-based genotype detection assay, which is capable of discerning nine distinct lactococcal genotypes to characterize these undefined starter cultures, and which can be applied to monitor compositiona

SUBMITTER: White K 

PROVIDER: S-EPMC10952461 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature