Identification and characterization of the TetR family transcriptional regulator NffT in Rhizobium johnstonii.

ABSTRACT: Symbiotic nitrogen fixation (SNF) by rhizobia is not only the main natural bionitrogen-source for organisms but also a green process leveraged to increase the fertility of soil for agricultural production. However, an insufficient understanding of the regulatory mechanism of SNF hinders its practical application. During SNF, nifA-fixA signaling is essential for the biosynthesis of nitrogenases and electron transfer chain proteins. In the present study, the TetR regulator NffT, whose mutation increased fixA expression, was discovered through a fixA-promoter-β-glucuronidase fusion assay performed with Rhizobium johnstonii. Real-time quantitative PCR analysis showed that nffT deletion increased the expression of symbiotic genes including nifA and f

SUBMITTER: Li X 

PROVIDER: S-EPMC10952539 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature