An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.

ABSTRACT: Land plants comprise two large monophyletic lineages, the vascular plants and the bryophytes, which diverged from their most recent common ancestor approximately 480 million years ago. Of the three lineages of bryophytes, only the mosses and the liverworts are systematically investigated, while the hornworts are understudied. Despite their importance for understanding fundamental questions of land plant evolution, they only recently became amenable to experimental investigation, with Anthoceros agrestis being developed as a hornwort model system. Availability of a high-quality genome assembly and a recently developed genetic transformation technique makes A. agrestis an attractive model species for hornworts. Here we describe an updated and optimized transformation protocol for A. agrestis

SUBMITTER: Waller M 

PROVIDER: S-EPMC10952725 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature