An enhancer trap system to track developmental dynamics in Marchantia polymorpha.

ABSTRACT: A combination of streamlined genetics, experimental tractability and relative morphological simplicity compared to vascular plants makes the liverwort Marchantia polymorpha an ideal model system for studying many aspects of plant biology. Here we describe a transformation vector combining a constitutive fluorescent membrane marker with a nuclear marker that is regulated by nearby enhancer elements and use this to produce a library of enhancer trap lines for Marchantia. Screening gemmae from these lines allowed the identification and characterization of novel marker lines, including markers for rhizoids and oil cells. The library allowed the identification of a margin tissue running around the thallus edge, highlighted during thallus development. The expression of this marker is correlated

SUBMITTER: Marron AO 

PROVIDER: S-EPMC10952768 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature