Transcription factor combinations that define human astrocyte identity encode significant variation of maturity and function.

ABSTRACT: Increasing evidence indicates that cellular identity can be reduced to the distinct gene regulatory networks controlled by transcription factors (TFs). However, redundancy exists in these states as different combinations of TFs can induce broadly similar cell types. We previously demonstrated that by overcoming gene silencing, it is possible to deterministically reprogram human pluripotent stem cells directly into cell types of various lineages. In the present study we leverage the consistency and precision of our approach to explore four different TF combinations encoding astrocyte identity, based on previously published reports. Analysis of the resulting induced astrocytes (iAs) demonstrated that all four cassettes generate cells with the typical morphology of in vitro astrocytes, which

SUBMITTER: Baranes K 

PROVIDER: S-EPMC10952910 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature