Cross-reactive CD8⁺ T cell responses to tumor-associated antigens (TAAs) and homologous microbiota-derived antigens (MoAs).

ABSTRACT:

Background

We have recently shown extensive sequence and conformational homology between tumor-associated antigens (TAAs) and antigens derived from microorganisms (MoAs). The present study aimed to assess the breadth of T-cell recognition specific to MoAs and the corresponding TAAs in healthy subjects (HS) and patients with cancer (CP).

Method

A library of > 100 peptide-MHC (pMHC) combinations was used to generate DNA-barcode labelled multimers. Homologous peptides were selected from the Cancer Antigenic Peptide Database, as well as Bacteroidetes/Firmicutes-derived peptides. They were incubated with CD8 + T cells from the peripheral blood of HLA-A*02:01 healthy individuals (n = 10) and cancer patients (n = 16). T cell recognition was identified using tetramer-staining analys

SUBMITTER: Cavalluzzo B 

PROVIDER: S-EPMC10953141 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature