Quantitative proteomic analysis of HER2 protein expression in PDAC tumors.

ABSTRACT: Metastatic pancreatic adenocarcinoma (PDAC) is the third leading cause of cancer-related death in the United States, with a 5-year survival rate of only 11%, necessitating identification of novel treatment paradigms. Tumor tissue specimens from patients with PDAC, breast cancer, and other solid tumor malignancies were collected and tumor cells were enriched using laser microdissection (LMD). Reverse phase protein array (RPPA) analysis was performed on enriched tumor cell lysates to quantify a 32-protein/phosphoprotein biomarker panel comprising known anticancer drug targets and/or cancer-related total and phosphorylated proteins, including HER2^Total, HER2^Y1248, and HER3^Y1289. RPPA analysis revealed significant levels of HER2^Total in PDAC patients

SUBMITTER: Randall J 

PROVIDER: S-EPMC10953162 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature