In vitro sepsis up-regulates Nociceptin/Orphanin FQ receptor expression and function on human T- but not B-cells.

ABSTRACT:

Background and purpose

In animal models of sepsis, increased activation of the Nociceptin/Orphanin FQ (N/OFQ) receptor NOP is associated with mortality and NOP antagonists improved survival. We have explored the role of the N/OFQ-NOP system in freshly isolated volunteer human B- and T-cells incubated with lipopolysaccharide (LPS) and peptidoglycan G (PepG) as a model of in vitro sepsis.

Experimental approach

B- and T-cell NOP expression was measured using the NOP fluorescent probe N/OFQ_ATTO594 , N/OFQ content was measured using immunofluorescence, N/OFQ release was tracked using a CHO_hNOPGαiq5 biosensor assay and NOP function was measured using transwell migration and cytokine/chemokine release using a 25-plex assay format. Cells were challenged with LP

SUBMITTER: Bird MF 

PROVIDER: S-EPMC10953342 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature