On-line targeted metabolomics for real-time monitoring of relevant compounds in fermentation processes.

ABSTRACT: Fermentation monitoring is a powerful tool for bioprocess development and optimization. On-line metabolomics is a technology that is starting to gain attention as a bioprocess monitoring tool, allowing the direct measurement of many compounds in the fermentation broth at a very high time resolution. In this work, targeted on-line metabolomics was used to monitor 40 metabolites of interest during three Escherichia coli succinate production fermentation experiments every 5 min with a triple quadrupole mass spectrometer. This allowed capturing high-time resolution biological data that can provide critical information for process optimization. For nine of these metabolites, simple univariate regression models were used to model compound concentration from their on-line mass spectrometry peak a

SUBMITTER: Cortada-Garcia J 

PROVIDER: S-EPMC10953439 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature