Single-Cell Sequencing Reveals Functional Alterations in Tuberculosis.

ABSTRACT: Despite its importance, the functional heterogeneity surrounding the dynamics of interactions between mycobacterium tuberculosis and human immune cells in determining host immune strength and tuberculosis (TB) outcomes, remains far from understood. This work now describes the development of a new technological platform to elucidate the immune function differences in individuals with TB, integrating single-cell RNA sequencing and cell surface antibody sequencing to provide both genomic and phenotypic information from the same samples. Single-cell analysis of 23 990 peripheral blood mononuclear cells from a new cohort of primary TB patients and healthy controls enables to not only show four distinct immune phenotypes (TB, myeloid, and natural killer (NK) cells), but also determine the dynami

SUBMITTER: Lyu M 

PROVIDER: S-EPMC10953544 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature