Fibronectin Connecting Cell Sheet Based on Click Chemistry for Wound Repair.

ABSTRACT: As a living repair material, cell sheet exhibits significant potential in wound repair. Nonetheless, wound healing is a complicated and protracted process that necessitates specific repair functions at each stage, including hemostasis and antibacterial activity. In this work, on the basis of harvesting the cell sheet via a photothermal response strategy, a fibronectin attached cell sheet (FACS) is prepared to enhance its wound repair capability. For this purpose, the azide group (N₃ ) is initially tagged onto the cell surface through metabolic glycoengineering of unnatural sugars, and then the conjugate (DBCO-fibronectin) comprises of the dibenzocyclooctyne (DBCO) and fibronectin with multiple wound repair functions is linked to N₃ using click chemistry. Biological ev

SUBMITTER: Xu W 

PROVIDER: S-EPMC10953575 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature