Analysis of <i>Microcystis aeruginosa</i> physiology by spectral flow cytometry: Impact of chemical and light exposure.

ABSTRACT: M. aeruginosa fluorescent changes were observed using a Cytek Aurora spectral flow cytometer that contains 5 lasers and 64 narrow band detectors located between 365 and 829 nm. Cyanobacteria were treated with different concentrations of H₂O₂ and then monitored after exposure between 1 and 8 days. The red fluorescence emission derived from the excitation of cyanobacteria with a yellow green laser (550 nm) was measured in the 652-669 nm detector while green fluorescence from excitation with a violet laser (405 nm) was measured in the 532-550 nm detector. The changes in these parameters were measured after the addition of H₂O₂. There was an initial increase in red fluorescence intensity at 24 hours. This was followed by a daily decreas

SUBMITTER: Brentjens ET 

PROVIDER: S-EPMC10953801 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature