Cryogenic enrichment of Plasmodium falciparum gametocytes from spiked whole blood.

ABSTRACT: Each individual cell type typically requires a unique set of conditions for optimal cryopreservation outcome, which relates to its specific response to cryoprotective agent (CPA) toxicity, osmotic behavior and sensitivity to ice crystallization. Cryopreservation of heterogenous cell populations is therefore exceedingly difficult as it requires separate and often conflicting conditions for each cell type. Conversely, these contrasting conditions could be utilized to favor cryogenic preference of a single cell population within a heterogenous sample, leading to its enrichment by elimination of remaining cells. To establish proof-of-concept for this overall approach, a protocol was developed for the cryogenic enrichment of Plasmodium falciparum gametocytes from whole blood. To accomplish this

SUBMITTER: Nesbitt JE 

PROVIDER: S-EPMC10954416 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature