A modular approach to enhancing cell membrane-coated nanoparticle functionality using genetic engineering.

ABSTRACT: Since their initial development, cell membrane-coated nanoparticles (CNPs) have become increasingly popular in the biomedical field. Despite their inherent versatility and ability to enable complex biological applications, there is considerable interest in augmenting the performance of CNPs through the introduction of additional functionalities. Here we demonstrate a genetic-engineering-based modular approach to CNP functionalization that can encompass a wide range of ligands onto the nanoparticle surface. The cell membrane coating is engineered to express a SpyCatcher membrane anchor that can readily form a covalent bond with any moiety modified with SpyTag. To demonstrate the broad utility of this technique, three unique targeted CNP formulations are generated using different classes of

SUBMITTER: Krishnan N 

PROVIDER: S-EPMC10954421 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature