METTL3-dependent m⁶A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.

ABSTRACT: Craniofacial malformations, often associated with syndromes, are prevalent birth defects. Emerging evidence underscores the importance of m⁶A modifications in various bioprocesses such as stem cell differentiation, tissue development, and tumorigenesis. Here, in vivo, experiments with zebrafish models revealed that mettl3-knockdown embryos at 144 h postfertilization exhibited aberrant craniofacial features, including altered mouth opening, jaw dimensions, ethmoid plate, tooth formation and hypoactive behavior. Similarly, low METTL3 expression inhibited the proliferation and migration of BMSCs, HEPM cells, and DPSCs. Loss of METTL3 led to reduced mRNA m⁶A methylation and PSEN1 expression, impacting craniofacial phenotypes. Co-injection of mettl3 or psen1 mRNA rescued t

SUBMITTER: Ma L 

PROVIDER: S-EPMC10954657 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature