Anion exchange HPLC monitoring of mRNA in vitro transcription reactions to support mRNA manufacturing process development.

ABSTRACT: mRNA technology has recently demonstrated the ability to significantly change the timeline for developing and delivering a new vaccine from years to months. The potential of mRNA technology for rapid vaccine development has recently been highlighted by the successful development and approval of two mRNA vaccines for COVID-19. Importantly, this RNA-based approach holds promise for treatments beyond vaccines and infectious diseases, e.g., treatments for cancer, metabolic disorders, cardiovascular conditions, and autoimmune diseases. There is currently significant demand for the development of improved manufacturing processes for the production of mRNA therapeutics in an effort to increase their yield and quality. The development of suitable analytical methods for the analysis of mRNA therape

SUBMITTER: Welbourne EN 

PROVIDER: S-EPMC10955092 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature