Mechanical loading of joint modulates T cells in lymph nodes to regulate osteoarthritis.

ABSTRACT:

Objective

The crosstalk of joint pathology with local lymph nodes in osteoarthritis (OA) is poorly understood. We characterized the change in T cells in lymph nodes following load-induced OA and established the association of the presence and migration of T cells to the onset and progression of OA.

Methods

We used an in vivo model of OA to induce mechanical load-induced joint damage. After cyclic tibial compression of mice, we analyzed lymph nodes for T cells using flow cytometry and joint pathology using histology and microcomputed tomography. The role of T-cell migration and the presence of T-cell type was examined using T-cell receptor (TCR)α-/- mice and an immunomodulatory drug, Sphingosine-1-phosphate (S1P) receptor inhibitor-treated mice, respectively.

Results

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SUBMITTER: Wheeler TA 

PROVIDER: S-EPMC10955501 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature