Dataset Information

Role of αENaC in root resorption of adjacent teeth due to entirely impacted mandibular third molars.

ABSTRACT:

Background

Entirely impacted mandibular third molar (EIM3M) concerns the pathological external root resorption (ERR) of the adjacent mandibular second molar (M2M) and formation of granulation tissue between two molars. The study aimed to clarify the effect of αENaC, a mechano-sensitive molecule, to explore the mechanical mechanism in this scenario.

Methods

The force EIM3M exerted on M2M was proved by finite element analysis. αENaC expressions were tested by real-time polymerase chain reaction (PCR), immunoblotting and immunofluorescence. Inflammatory and epithelial-mesenchymal transition (EMT)-related molecules expressions were also detected by real-time PCR. The correlation was analyzed by Spearman's correlation analysis, and receiver-operator characteristic (ROC) curve was further exhibited.

Results

The force was concentrated in the ERR area. αENaC was upregulated, positively correlated with ERR degree and localized to the fibroblasts in ERR granulation tissues. Moreover, αENaC was respectively and positively associated with elevated TNF-α and N-cadherin in ERR granulation tissues. More importantly, ROC analysis verified αENaC as a novel indication of the incidence of this disease.

Conclusions

Our finding revealed the force from EIM3M causing ERR of M2M, and elucidated the expression and localization of αENaC and its positive correlation with inflammation, EMT and disease severity, suggesting a novel indication in this disease.

SUBMITTER: Tang J

PROVIDER: S-EPMC10956368 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Publications

Role of αENaC in root resorption of adjacent teeth due to entirely impacted mandibular third molars.

Tang Jiaqi J Yu Weijun W Lin Lu L Yang Ruhan R Li Guanglong G Jin Min M Gu Yuting Y Jiang Bin B Lu Eryi E

BMC oral health 20240321 1

<h4>Background</h4>Entirely impacted mandibular third molar (EIM3M) concerns the pathological external root resorption (ERR) of the adjacent mandibular second molar (M2M) and formation of granulation tissue between two molars. The study aimed to clarify the effect of αENaC, a mechano-sensitive molecule, to explore the mechanical mechanism in this scenario.<h4>Methods</h4>The force EIM3M exerted on M2M was proved by finite element analysis. αENaC expressions were tested by real-time polymerase ch ...[more]

PMID: 38515079

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Project description:ObjectivesThe aim of this study is to investigate risk factors for external root resorption (ERR) of second molars (M2) associated with impacted third molars (M3), and to develop a prediction model that can offer dentists a reliable and efficient tool for predicting the likelihood of ERR.MethodsA total of 798 patients with 2156 impacted third molars were collected from three centres between 1 December 2018 and 15 December 2018. ERR was identified by cone beam computed tomography examinations. The effects of different risk factors on the presence/absence of ERR and its severity were analysed using Chi-square or Fisher test. Multivariate logistic regressive analysis with stepwise variable selection methods was performed to identify factors which were significant predictors for ERR and its severity. Subsequently, a prediction model was developed, and the model performance was validated internally and externally.ResultsThe overall incidence of ERR of second molars was 16.05%. The prediction model was established using six factors including position (upper/lower jaw), impact type, impact depth (PG: A-B-C), contact position, root number of M3, and age. In terms of internal validation, the prediction model demonstrated satisfactory performance, achieving an area under curve of 0.961 and a prediction accuracy of 0.907. As for external validation, the area under curve remained high at 0.953, with a prediction accuracy of 0.892.ConclusionA risk prediction model for ERR was established in the present study. Position (upper or lower jaw), impact type, impact depth (PG: A-B-C), contact position, root number of M3, and age were identified as influencing variables which were significant predictors in the development of this predictive model. The prediction model showed great discrimination and calibration.Clinical relevanceThis prediction model has the potential to aid dentists and patients in making clinical decisions regarding the necessity of M3 extraction.

Project description:Study designAutotransplantation, if possible, is a viable option for replacing a missing tooth when a donor tooth is available. The most typical tooth transplant is the transfer of a third molar to a first molar site. No immune reaction results from transplants of this nature. It restores the proprioceptive function and normal periodontal healing; thus, the patient can have a natural chewing feeling and natural biological response.ObjectiveThis study aims to evaluate the prognosis of autotransplanted mandibular third molar and also to evaluate the cost effectiveness of the treatment performed when compared to the other treatment modalities for prosthetic rehabilitation.MethodsA prospective study was done in the Department of Oral & Maxillofacial Surgery, Sardar Patel Post Graduate Institute of Dental & Medical Sciences, Lucknow, UP, India, with over 20 patients to evaluate the prognosis of autotransplanted mandibular third molars with complete root formation after atraumatic extraction of first or second mandibular molar, which were randomly selected irrespective of race, sex, caste, and socio-economic status. Regular clinical and radiographical examinations were performed over a period of 1 year and the patients were assessed for pain, swelling, infection, dry socket, periodontal pocket depth, ankylosis, root resorption, tooth mobility, and level of buccal bone in relation to cementoenamel junction (CEJ).ResultsEighteen out of 20 transplants were successful; only 2 mandibular transplants were extracted because of abnormal horizontal and axial mobility and the reason of failure was attributed to fact that the roots of transplant were short and conical and there was lack of alveolar bone height at the recipient site in one patient, while root resorption was the reason for failure of transplant in the other patient.ConclusionsThis study assessed the efficacy of autotransplantation of molars and the viability of the procedure to replace unrestorable molar teeth; it also supports the hypothesis that transplantation of a mandibular third molar for replacement of a lost or seriously damaged molar tooth could be a reasonable alternative.

Dataset Information

Role of αENaC in root resorption of adjacent teeth due to entirely impacted mandibular third molars.

Background

Methods

Results

Conclusions

Publications

Role of αENaC in root resorption of adjacent teeth due to entirely impacted mandibular third molars.

Similar Datasets

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