Application of enzyme-linked immunosorbent assay to detect antimicrobial peptides in human intestinal lumen.

ABSTRACT: Intestinal transplantation is the definitive treatment for intestinal failure. However, tissue rejection and graft-versus-host disease are relatively common complications, necessitating aggressive immunosuppression that can itself pose further complications. Tracking intraluminal markers in ileal effluent from standard ileostomies may present a noninvasive and sensitive way to detect developing pathology within the intestinal graft. This would be an improvement compared to current assessments, which are limited by poor sensitivity and specificity, contributing to under or over-immunosuppression, respectively, and by the need for invasive biopsies. Herein, we report an approach to reproducibly analyze ileal fluid obtained through stoma sampling for antimicrobial peptide/protein concentratio

SUBMITTER: Hong JS 

PROVIDER: S-EPMC10956375 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature