Characteristics of intestinal microbiota in preterm infants and the effects of probiotic supplementation on the microbiota.

ABSTRACT:

Objective

In this study, we investigated the characteristics of the intestinal microbiota of preterm infants, and then analyzed the effects of probiotics supplementation on intestinal microbiota in preterm infants.

Methods

This study enrolled 64 infants born between 26 and 32 weeks gestational age (GA) and 22 full-term infants. 34 premature infants received oral probiotic supplementation for 28 days. Stool samples were obtained on the first day (D1) and the 28th day (D28) after birth for each infant. Total bacterial DNA was extracted and sequenced using the Illumina MiSeq Sequencing System, specifically targeting the V3-V4 hyper-variable regions of the 16S rDNA gene. The sequencing results were then used to compare and analyze the composition and diversity index of the intes

SUBMITTER: Yang S 

PROVIDER: S-EPMC10956575 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature