Accessible luminal interface of bovine rectal organoids generated from cryopreserved biopsy tissues.

ABSTRACT: Developing precise species-specific in vitro models that closely resemble in vivo intestinal tissues is essential for advancing our understanding of gastrointestinal physiology and associated diseases. This is especially crucial in examining host-pathogen interactions, particularly in bovines, a known reservoir for microbes and pathogens posing substantial public health threats. This research investigated the viability of producing bovine rectal organoids from cryopreserved tissues. We compared two cryopreservation methods with a traditional technique using fresh tissues, evaluating their effectiveness through growth rates, long-term viability, and comprehensive structural, cellular, and genetic analyses. These assessments utilized phase-contrast imaging, immunofluorescence imaging, and RT

SUBMITTER: Kawasaki M 

PROVIDER: S-EPMC10956885 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature