Validation of a size exclusion method for concomitant purification and formulation of peptide radiopharmaceuticals.

ABSTRACT:

Background

Both in clinical routine and in preclinical research, the established standard procedure for the final purification of radiometal-labeled peptide radiopharmaceuticals is cartridge-based reversed-phase (RP) solid phase extraction (SPE). It allows the rapid and quantitative separation of the radiolabeled peptide from hydrophilic impurities and easy integration into automated synthesis procedures. However, product elution from RP cartridges necessitates the use of organic solvents and product recovery is sometimes limited. Thus, an alternative purification method based on commercially available size exclusion cartridges was investigated.

Results

Since most peptide radiopharmaceuticals have a molecular weight > 1 kDa, Sephadex G10 cartridges with a molecular size cut-

SUBMITTER: Martin S 

PROVIDER: S-EPMC10957824 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature