Dataset Information

Author Correction: Unraveling the epigenetic code: human kidney DNA methylation and chromatin dynamics in renal disease development.

ABSTRACT:

SUBMITTER: Yan Y

PROVIDER: S-EPMC10958046 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Author Correction: Unraveling the epigenetic code: human kidney DNA methylation and chromatin dynamics in renal disease development.

Yan Yu Y Liu Hongbo H Abedini Amin A Sheng Xin X Palmer Matthew M Li Hongzhe H Susztak Katalin K

Nature communications 20240321 1

PMID: 38514613

Similar Datasets

Project description:Spermatogonial stem cells (SSCs) are able to undergo both self-renewal and differentiation. Unlike self-renewal, which replenishes the SSC and progenitor pool, differentiation is an irreversible process committing cells to meiosis. Although the preparations for meiotic events in differentiating spermatogonia (Di-SG) are likely to be accompanied by alterations in chromatin structure, the three-dimensional chromatin architectural differences between SSCs and Di-SG, and the higher-order chromatin dynamics during spermatogonial differentiation, have not been systematically investigated. Here, we performed in situ high-throughput chromosome conformation capture, RNA-seq, and chromatin immunoprecipitation-sequencing analyses on porcine undifferentiated spermatogonia (which consist of SSCs and progenitors) and Di-SG. We identified that Di-SG exhibited less compact chromatin structural organization, weakened compartmentalization, and diminished topologically associating domains in comparison with undifferentiated spermatogonia, suggesting that diminished higher-order chromatin architecture in meiotic cells, as shown by recent reports, might be preprogrammed in Di-SG. Our data also revealed that A/B compartments, representing open or closed chromatin regions respectively, and topologically associating domains were related to dynamic gene expression during spermatogonial differentiation. Furthermore, we unraveled the contribution of promoter-enhancer interactions to premeiotic transcriptional regulation, which has not been accomplished in previous studies due to limited cell input and resolution. Together, our study uncovered the three-dimensional chromatin structure of SSCs/progenitors and Di-SG, as well as the interplay between higher-order chromatin architecture and dynamic gene expression during spermatogonial differentiation. These findings provide novel insights into the mechanisms for SSC self-renewal and differentiation and have implications for diagnosis and treatment of male sub-/infertility.

Dataset Information

Author Correction: Unraveling the epigenetic code: human kidney DNA methylation and chromatin dynamics in renal disease development.

Publications

Author Correction: Unraveling the epigenetic code: human kidney DNA methylation and chromatin dynamics in renal disease development.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets