Acylcarnitines promote gallbladder cancer metastasis through lncBCL2L11-THOC5-JNK axis.

ABSTRACT:

Background

The progression of gallbladder cancer (GBC) is accompanied by abnormal fatty acid β-oxidation (FAO) metabolism. Different types of lipids perform various biological functions. This study aimed to determine the role of acyl carnitines in the molecular mechanisms of GBC progression.

Methods

Distribution of lipids in GBC was described by LC-MS-based lipidomics. Cellular localization, expression level and full-length of lncBCL2L11 were detected using fluorescence in situ hybridization (FISH) assays, subcellular fractionation assay and 5' and 3' rapid amplification of the cDNA ends (RACE), respectively. In vitro and in vivo experiments were used to verify the biological function of lncBCL2L11 in GBC cells. Methylated RNA Immunoprecipitation (MeRIP) was performed to det

SUBMITTER: Yang Y 

PROVIDER: S-EPMC10958842 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature