Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation.

ABSTRACT: Partial reprogramming by cyclic short-term expression of Yamanaka factors holds promise for shifting cells to younger states and consequently delaying the onset of many diseases of aging. However, the delivery of transgenes and potential risk of teratoma formation present challenges for in vivo applications. Recent advances include the use of cocktails of compounds to reprogram somatic cells, but the characteristics and mechanisms of partial cellular reprogramming by chemicals remain unclear. Here, we report a multi-omics characterization of partial chemical reprogramming in fibroblasts from young and aged mice. We measured the effects of partial chemical reprogramming on the epigenome, transcriptome, proteome, phosphoproteome, and metabolome. At the transcriptome, proteome, and phosphopro

SUBMITTER: Mitchell W 

PROVIDER: S-EPMC10959535 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature