Predicting nuclear G-quadruplex RNA-binding proteins with roles in transcription and phase separation.

ABSTRACT: RNA-binding proteins are central for many biological processes and their characterization has demonstrated a broad range of functions as well as a wide spectrum of target structures. RNA G-quadruplexes are important regulatory elements occurring in both coding and non-coding transcripts, yet our knowledge of their structure-based interactions is at present limited. Here, using theoretical predictions and experimental approaches, we show that many chromatin-binding proteins bind to RNA G-quadruplexes, and we classify them based on their RNA G-quadruplex-binding potential. Combining experimental identification of nuclear RNA G-quadruplex-binding proteins with computational approaches, we build a prediction tool that assigns probability score for a nuclear protein to bind RNA G-quadruplexes.

SUBMITTER: Luige J 

PROVIDER: S-EPMC10959947 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature