A multiplex DNA probe-based method for simultaneous identification of adulteration in meat samples.

ABSTRACT: Meat adulteration and admixing are prevalent malpractices observed in processed and raw meat samples, where the consumption of adulterated meat has been associated with food allergies, financial losses, and consumer distrust. Meat authentication is pivotal to address these concerns. The meat authenticity can be determined through genetic, protein, and immunological markers and advanced detection methods. However, these methods often target a single species and lack the specificity to distinguish closely related species. Here, in the present study, we have developed a multiplex detection method based on the species-specific primers and probes, that can target four meat species in one reaction. The developed method amplifies the mitochondrial genomic regions of chicken, pork, sheep and goat

SUBMITTER: Singh Yadav S 

PROVIDER: S-EPMC10960131 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature