Adsorption of Hg²⁺/Cr⁶⁺ by metal-binding proteins heterologously expressed in Escherichia coli.

ABSTRACT:

Background

Removal of heavy metals from water and soil is a pressing challenge in environmental engineering, and biosorption by microorganisms is considered as one of the most cost-effective methods. In this study, the metal-binding proteins MerR and ChrB derived from Cupriavidus metallidurans were separately expressed in Escherichia coli BL21 to construct adsorption strains. To improve the adsorption performance, surface display and codon optimization were carried out.

Results

In this study, we constructed 24 adsorption engineering strains for Hg²⁺ and Cr⁶⁺, utilizing different strategies. Among these engineering strains, the M'-002 and B-008 had the strongest heavy metal ion absorption ability. The M'-002 used the flexible linker and INPN to display t

SUBMITTER: Hu S 

PROVIDER: S-EPMC10960487 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature