High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models.

ABSTRACT: Fluorescence nanosilica-based cell tracker has been explored and applied in cell biological research. However, the aggregation of these nanoparticles at physiological pH is still the main limitation. In this research, we introduced a novel fluorescence nano-based cell tracker suitable for application in live cells. The silica-coated fluorescein isothiocyanate isomer (FITC-SiO₂) nanoparticles (NPs) were modified with carboxymethylsilanetriol disodium salt (FITC-SiO₂-COOH), integrating the dianion form of FITC molecules. This nanosystem exhibited superior dispersion in aqueous solutions and effectively mitigated dye leakage. These labeled NPs displayed notable biocompatibility and minimal cytotoxicity in both in vitro and in vivo conditions. Significantly, the NPs did n

SUBMITTER: Nguyen TT 

PROVIDER: S-EPMC10960792 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature