Force and kinetics of fast and slow muscle myosin determined with a synthetic sarcomere-like nanomachine.

ABSTRACT: Myosin II is the muscle molecular motor that works in two bipolar arrays in each thick filament of the striated (skeletal and cardiac) muscle, converting the chemical energy into steady force and shortening by cyclic ATP-driven interactions with the nearby actin filaments. Different isoforms of the myosin motor in the skeletal muscles account for the different functional requirements of the slow muscles (primarily responsible for the posture) and fast muscles (responsible for voluntary movements). To clarify the molecular basis of the differences, here the isoform-dependent mechanokinetic parameters underpinning the force of slow and fast muscles are defined with a unidimensional synthetic nanomachine powered by pure myosin isoforms from either slow or fast rabbit skeletal muscle. Data fit

SUBMITTER: Buonfiglio V 

PROVIDER: S-EPMC10960843 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature