Enhanced bacterial cancer therapy delivering therapeutic RNA interference of c-Myc.

ABSTRACT:

Background

Bacterial cancer therapy was first trialled in patients at the end of the nineteenth century. More recently, tumour-targeting bacteria have been harnessed to deliver plasmid-expressed therapeutic interfering RNA to a range of solid tumours. A major limitation to clinical translation of this is the short-term nature of RNA interference in vivo due to plasmid instability. To overcome this, we sought to develop tumour-targeting attenuated bacteria that stably express shRNA by virtue of integration of an expression cassette within the bacterial chromosome and demonstrate therapeutic efficacy in vitro and in vivo.

Results

The attenuated tumour targeting Salmonella typhimurium SL7207 strain was modified to carry chromosomally integrated shRNA expression cassettes at the

SUBMITTER: Williams JS 

PROVIDER: S-EPMC10961001 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature