Single-cell sequencing reveals the immune landscape of breast cancer patients with brain metastasis.

ABSTRACT:

Background

Breast cancer has the highest incidence rate of cancer worldwide, and brain metastases (BrM) are among the most malignant cases. While some patients have benefited from immune checkpoint inhibitors (ICIs), the complex anatomical structure of the brain and the heterogeneity of metastatic tumors have made it difficult to characterize the tumor immune microenvironment (TME) of metastatic tumors.

Methods

To address this, we used single-cell RNA sequencing (scRNA-seq) to analyze immune cells in the cerebrospinal fluid (CSF) of BrM patients with breast cancer, thereby providing a comprehensive view of the immune microenvironment landscape of BrM.

Results

Based on canonical marker genes, we identified nine cell types, and further identified their subtypes through

SUBMITTER: Zhou H 

PROVIDER: S-EPMC10961220 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature