Estimation of Peptide Helicity from Circular Dichroism Using the Ensemble Model.

ABSTRACT: An established method for the quantitation of the helix content in peptides using circular dichroism (CD) relies on the linear spectroscopic model. This model assumes an average value of the helix-length correction for all peptide conformers, irrespective of the length of the helical segment. Here we assess the validity of this approximation and introduce a more physically realistic ensemble-based analysis of the CD signal in which the length correction is assigned specifically to each ensemble conformer. We demonstrate that the linear model underestimates peptide helicity, with the difference depending on the ensemble composition. We developed a computer program that implements the ensemble model to estimate the peptide helicity. Using this model and the CD data set covering a broad range

SUBMITTER: Zavrtanik U 

PROVIDER: S-EPMC10961730 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature