Regulation of headache response and transcriptomic network by the trigeminal ganglion clock.

ABSTRACT:

Objective

To characterize the circadian features of the trigeminal ganglion in a mouse model of headache.

Background

Several headache disorders, such as migraine and cluster headache, are known to exhibit distinct circadian rhythms of attacks. The circadian basis for these rhythmic pain responses, however, remains poorly understood.

Methods

We examined trigeminal ganglion ex vivo and single-cell cultures from Per2::LucSV reporter mice and performed immunohistochemistry. Circadian behavior and transcriptomics were investigated using a novel combination of trigeminovascular and circadian models: a nitroglycerin mouse headache model with mechanical thresholds measured every 6 h, and trigeminal ganglion RNA sequencing measured every 4 h for 24 h. Finally, we performed pha

SUBMITTER: Han C 

PROVIDER: S-EPMC10961824 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature