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¹²⁹Xe Dynamic Nuclear Polarization Demystified: The Influence of the Glassing Matrix on the Radical Properties.

ABSTRACT: ¹²⁹Xe dissolution dynamic nuclear polarization (DNP) is a controversial topic. The gold standard technique for hyperpolarized xenon magnetic resonance imaging (MRI) is spin exchange optical pumping, which received FDA approval in 2022. Nevertheless, the versatility of DNP for enhancing the signal of any NMR active nucleus might provide new perspectives for hyperpolarized ¹²⁹Xe NMR/MRI. Initial publications about ¹²⁹Xe DNP underlined the increased complexity in the sample preparation and lower polarization levels when compared to more conventional ¹³C-labeled molecules, at same experimental conditions, despite very close gyromagnetic ratios. Herein, we introduce, using a Custom Fluid Path system, a user-friendly and very robust sample preparation method. Moreover, investigating the radical properties at real DNP conditions by means of LOngitudinal Detected Electron Spin Resonance, we discovered a dramatic shortening of the electron spin longitudinal relaxation time (T_1e) of nitroxyl radicals in xenon DNP samples' matrices, with respect to more commonly used water:glycerol ones. Mitigating those challenges through microwave frequency modulation, we achieved over 20% ¹²⁹Xe polarization without employing any deuterated solvent.

SUBMITTER: Wistrom E

PROVIDER: S-EPMC10961830 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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129Xe Dynamic Nuclear Polarization Demystified: The Influence of the Glassing Matrix on the Radical Properties.

Wiström Emma E Hyacinthe Jean-Noël JN Lê Thanh Phong TP Gruetter Rolf R Capozzi Andrea A

The journal of physical chemistry letters 20240307 11

129Xe dissolution dynamic nuclear polarization (DNP) is a controversial topic. The gold standard technique for hyperpolarized xenon magnetic resonance imaging (MRI) is spin exchange optical pumping, which received FDA approval in 2022. Nevertheless, the versatility of DNP for enhancing the signal of any NMR active nucleus might provide new perspectives for hyperpolarized 129Xe NMR/MRI. Initial publications about 129Xe DNP underlined the increased complexity in th ...[more]

PMID: 38453156

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Project description:Background129 Xe gas-transfer MRI provides regional measures of pulmonary gas exchange in adults and separates xenon in interstitial lung tissue/plasma (barrier) from xenon in red blood cells (RBCs). The technique has yet to be demonstrated in pediatric populations or conditions.Purpose/hypothesisTo perform an exploratory analysis of 129 Xe gas-transfer MRI in children.Study typeProspective.PopulationSeventy-seven human volunteers (38 males, age = 17.7 ± 15.1 years, range 5-68 years, 16 healthy). Four pediatric disease cohorts.Field strength/sequence3-T, three-dimensional-radial one-point Dixon Fast Field Echo (FFE) Ultrashort Echo Time (UTE).AssessmentBreath hold compliance was assessed by quantitative signal-to-noise and dynamic metrics. Whole-lung means and standard deviations were extracted from gas-transfer maps. Gas-transfer metrics were investigated with respect to age and lung disease. Clinical pulmonary function tests were retrospectively acquired for reference lung disease severity.Statistical testsWilcoxon rank-sum tests to compare age and disease cohorts, Wilcoxon signed-rank tests to compare pre- and post-breath hold vitals, Pearson correlations between age and gas-transfer metrics, and limits of normal with a binomial exact test to compare fraction of subjects with abnormal gas-transfer. P ≤ 0.05 was considered significant.ResultsEighty percentage of pediatric subjects successfully completed 129 Xe gas-transfer MRI. Gas-transfer parameters differed between healthy children and adults, including ventilation (0.75 and 0.67) and RBC:barrier ratio (0.31 and 0.46) which also correlated with age (ρ = -0.76, 0.57, respectively). Bone marrow transplant subjects had impaired ventilation (90% of reference) and increased dissolved 129 Xe standard deviation (242%). Bronchopulmonary dysplasia subjects had decreased barrier-uptake (69%). Cystic fibrosis subjects had impaired ventilation (91%) and increased RBC-transfer (146%). Lastly, childhood interstitial lung disease subjects had increased ventilation heterogeneity (113%). Limits of normal provided detection of abnormalities in additional gas-transfer parameters.Data conclusionPediatric 129 Xe gas-transfer MRI was adequately successful and gas-transfer metrics correlated with age. Exploratory analysis revealed abnormalities in a variety of pediatric obstructive and restrictive lung diseases.Level of evidence2 TECHNICAL EFFICACY STAGE: 2.

Dataset Information

¹²⁹Xe Dynamic Nuclear Polarization Demystified: The Influence of the Glassing Matrix on the Radical Properties.

Publications

<sup>129</sup>Xe Dynamic Nuclear Polarization Demystified: The Influence of the Glassing Matrix on the Radical Properties.

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