Single-cell transcriptional signature-based drug repurposing and in vitro evaluation in colorectal cancer.

ABSTRACT:

Background

The need for intelligent and effective treatment of diseases and the increase in drug design costs have raised drug repurposing as one of the effective strategies in biomedicine. There are various computational methods for drug repurposing, one of which is using transcription signatures, especially single-cell RNA sequencing (scRNA-seq) data, which show us a clear and comprehensive view of the inside of the cell to compare the state of disease and health.

Methods

In this study, we used 91,103 scRNA-seq samples from 29 patients with colorectal cancer (GSE144735 and GSE132465). First, differential gene expression (DGE) analysis was done using the ASAP website. Then we reached a list of drugs that can reverse the gene signature pattern from cancer to normal using the

SUBMITTER: Mahdi-Esferizi R 

PROVIDER: S-EPMC10962075 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature