Neutrophil Extracellular Traps and Thrombolysis Resistance: New Insights for Targeting Therapies.

ABSTRACT:

Background

Thrombosis is linked to neutrophil release of neutrophil extracellular traps (NETs). NETs are proposed as a mechanism of resistance to thrombolysis. This study intends to analyze the composition of thrombi retrieved after mechanical thrombectomy, estimate the age and organization of thrombi, and evaluate associations with the use of thrombolysis, antiplatelets, and heparin.

Methods

This retrospective observational study involved 72 samples (44 from cerebral and 28 coronary arteries), which were stained with hematoxylin and eosin, anti-NE (neutrophil elastase) antibody, and anti-histone H2B (histone H2B) antibody, representing different components in NET formation, all detectable during the later stages of NETosis, for histochemical and digital quantification of NE

SUBMITTER: Mengozzi L 

PROVIDER: S-EPMC10962437 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature