Gold-FISH enables targeted NanoSIMS analysis of plant-associated bacteria.

ABSTRACT: Bacteria colonize plant roots and engage in reciprocal interactions with their hosts. However, the contribution of individual taxa or groups of bacteria to plant nutrition and fitness is not well characterized due to a lack of in situ evidence of bacterial activity. To address this knowledge gap, we developed an analytical approach that combines the identification and localization of individual bacteria on root surfaces via gold-based in situ hybridization with correlative NanoSIMS imaging of incorporated stable isotopes, indicative of metabolic activity. We incubated Kosakonia strain DS-1-associated, gnotobiotically grown rice plants with ¹⁵ N-N₂ gas to detect in situ N₂ fixation activity. Bacterial cells along the rhizoplane showed heterogeneous patterns

SUBMITTER: Schmidt H 

PROVIDER: S-EPMC10962543 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature