Climbing fiber multi-innervation of mouse Purkinje dendrites with arborization common to human.

ABSTRACT: Canonically, each Purkinje cell (PC) in the adult cerebellum receives only one climbing fiber (CF) from the inferior olive. Underlying current theories of cerebellar function is the notion that this highly conserved one-to-one relationship renders Purkinje dendrites into a single computational compartment. However, we discovered that multiple primary dendrites are a near-universal morphological feature in humans. Using tract tracing, immunolabeling, and in vitro electrophysiology, we found that in mice ~25% of mature multibranched cells receive more than one CF input. Two-photon calcium imaging in vivo revealed that separate dendrites can exhibit distinct response properties to sensory stimulation, indicating that some multibranched cells integrate functionally independent CF-receptive fie

SUBMITTER: Busch SE 

PROVIDER: S-EPMC10962609 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature