An auxiliary strategy of partial least squares regression in pharmacokinetic/pharmacodynamic studies: A case of application of guhong injection in myocardial ischemia/reperfusion rats.

ABSTRACT: Guhong injection (GHI) has been applied in the therapy of cardio-cerebrovascular disease in clinic, but there is no report about the pharmacokinetic/pharmacodynamic (PK/PD) research on GHI treating myocardial ischemia/reperfusion (MI/R) injury in rats. In this study, eight compounds of GHI in plasma, including N-acetyl-L-glutamine (NAG), chlorogenic acid (CGA), hydroxysafflor yellow A (HSYA), p-coumaric acid ( pCA), rutin, hyperoside, kaempferol-3-O-rutinoside, and kaempferol-3-O-glucoside, were quantified by LC-MS/MS. We discovered that the values of t_1/2β, k₁₂, V₂, and CL₂ were larger than those of t_1/2α, k₂₁, V₁, and CL₁ for all compounds. The levels of four biomarkers, creatine kinase-MB (CK-MB), ca

SUBMITTER: Chen HY 

PROVIDER: S-EPMC10962654 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature