Impact of eIF2α phosphorylation on the translational landscape of mouse embryonic stem cells.

ABSTRACT: The integrated stress response (ISR) is critical for cell survival under stress. In response to diverse environmental cues, eIF2α becomes phosphorylated, engendering a dramatic change in mRNA translation. The activation of ISR plays a pivotal role in the early embryogenesis, but the eIF2-dependent translational landscape in pluripotent embryonic stem cells (ESCs) is largely unexplored. We employ a multi-omics approach consisting of ribosome profiling, proteomics, and metabolomics in wild-type (eIF2α^+/+) and phosphorylation-deficient mutant eIF2α (eIF2α^A/A) mouse ESCs (mESCs) to investigate phosphorylated (p)-eIF2α-dependent translational control of naive pluripotency. We show a transient increase in p-eIF2α in the naive epiblast layer of E4.5 embryos. Absence of eIF2α

SUBMITTER: Amiri M 

PROVIDER: S-EPMC10962698 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature