Thermodynamic modulation of gephyrin condensation by inhibitory synapse components.

ABSTRACT: Phase separation drives compartmentalization of intracellular contents into various biomolecular condensates. Individual condensate components are thought to differentially contribute to the organization and function of condensates. However, how intermolecular interactions among constituent biomolecules modulate the phase behaviors of multicomponent condensates remains unclear. Here, we used core components of the inhibitory postsynaptic density (iPSD) as a model system to quantitatively probe how the network of intra- and intermolecular interactions defines the composition and cellular distribution of biomolecular condensates. We found that oligomerization-driven phase separation of gephyrin, an iPSD-specific scaffold, is critically modulated by an intrinsically disordered linker region e

SUBMITTER: Lee G 

PROVIDER: S-EPMC10963017 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature