A maximum of two readily releasable vesicles per docking site at a cerebellar single active zone synapse.

ABSTRACT: Recent research suggests that in central mammalian synapses, active zones contain several docking sites acting in parallel. Before release, one or several synaptic vesicles (SVs) are thought to bind to each docking site, forming the readily releasable pool (RRP). Determining the RRP size per docking site has important implications for short-term synaptic plasticity. Here, using mouse cerebellar slices, we take advantage of recently developed methods to count the number of released SVs at single glutamatergic synapses in response to trains of action potentials (APs). In each recording, the number of docking sites was determined by fitting with a binomial model the number of released SVs in response to individual APs. After normalization with respect to the number of docking sites, the summe

SUBMITTER: Silva M 

PROVIDER: S-EPMC10963025 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature