Notopterol mitigates IL-1β-triggered pyroptosis by blocking NLRP3 inflammasome via the JAK2/NF-kB/hsa-miR-4282 route in osteoarthritis.

ABSTRACT:

Objective

Osteoarthritis (OA), the most prevalent form of arthritis, impacts approximately 10% of men and 18% of women aged above 60 years. Currently, a complete cure for OA remains elusive, making clinical management challenging. The traditional Chinese herb Notopterygium incisum, integral to the Juanbi pill for rheumatism, shows promise in safeguarding chondrocytes through its strong anti-inflammatory effects.

Methods

To explore the protective effect of notopterol and miRNA (has-miR-4248) against inflammation, we simulated an inflammatory environment in chondrocytes cell lines C20A4 and C28/12, focusing on inflammasome formation and pyroptosis.

Results

Our finding indicates notopterol significantly reduced interleukin (IL)-18 and tumor necrosis factor (TNF)-a

SUBMITTER: Chen KT 

PROVIDER: S-EPMC10963379 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature