Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro.

ABSTRACT: In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. In addition, immunocytochemical labelling confirmed the detection of UBF in 4-cell FLI parthenogenic embryos, suggesting similarities with natural embryo development. Through RNAseq a

SUBMITTER: Rosenbaum Bartkova A 

PROVIDER: S-EPMC10963758 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature